Biofilm Removal using Phosphate Buffer Saline
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Abstract
Biofilm represents a serious threat to water, medical and health facilities. Dealing with such an engineering problem may be pre or post formation. As a rough speech, there is no pre-treatment method could stop biofilm formation completely while post treatment cost a lot of expenses and resources. This gives the potential to explore a method helping to remove and disinfect biofilms completely. In its quantification, Phosphate Buffer Saline (PBS) was used to release biofilm from coupons that mounted in various laboratory scale reactors. This sparks the idea of using this substance to remove biofilm after its foundation. To explore that, the two controlling variables of exposure time and PBS concentration were examined against biofilm percentage removal. Results were promising and gave a strong potential for practical and commercial use.
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A. Amod, A. A. Anand, A. K. Sahoo, and S. K. Samanta, "Diagnostic and therapeutic strategies in combating implanted medical device-associated bacterial biofilm infections," Folia Microbiologica, vol. 70, pp. 321-342, 2025/04/01 2025.
I. Hashem and J. F. Van Impe, "The territorial nature of aggression in biofilms," Frontiers in Microbiology, vol. 13, p. 878223, 2022.
Z. Lewandowski and H. Beyenal, Fundamentals of biofilm research: CRC press, 2013.
Y. Liu, S. Long, H. Wang, and Y. Wang, "Biofilm therapy for chronic wounds," International Wound Journal, vol. 21, p. e14667, 2024.
R. Sadeghzadeh, Z. Esfandiari, A. M. Khaneghah, and M. Rostami, "A Review of Challenges and Solutions of Biofilm Formation of Escherichia coli: Conventional and Novel Methods of Prevention and Control," Food and Bioprocess Technology, vol. 17, pp. 2583-2618, 2024/09/01 2024.
K. Sauer, P. Stoodley, D. M. Goeres, L. Hall-Stoodley, M. Burmølle, P. S. Stewart, et al., "The biofilm life cycle: expanding the conceptual model of biofilm formation," Nature Reviews Microbiology, vol. 20, pp. 608-620, 2022.
S. S. Epstein, "Microbial awakenings," Nature, vol. 457, pp. 1083-1083, 2009.
Z. Lewandowski and H. Beyenal, Fundamentals of Biofilm Research, Second Edition: Taylor & Francis, 2013.
S. K. Shukla and T. S. Rao, "An Improved Crystal Violet Assay for Biofilm Quantification in 96-Well Microtitre Plate," bioRxiv, p. 100214, 2017.
J. Nilles, J. Weiss, and D. Theile, "Crystal Violet Staining is a Reliable Alternative to Bicinchoninic Acid Assay-Based Normalization," BioTechniques, vol. 73, pp. 131-135, 2022/09/01 2022.
G. Hassan, Q. A.-N. A. K. ALIbady, and A. K. Hashim, "Biofilm formation rate measurement in water and biomedical systems using photometric smartphones applications," AIP Conference Proceedings, vol. 3169, 2025.
N. Bouhrour, P. H. Nibbering, and F. Bendali, "Medical Device-Associated Biofilm Infections and Multidrug-Resistant Pathogens," Pathogens, vol. 13, p. 393, 2024.
R. Thakre, D. N. Bankar, Y. Tiwade, and V. Mishra, "The battle against biofilms: understanding the impact on medical devices and patient health," Reviews and Research in Medical Microbiology, vol. 36, 2025.
C. Wang, Y. Su, S. M. S. Shahriar, Y. Li, and J. Xie, "Emerging strategies for treating medical device and wound-associated biofilm infections," Microbial Biotechnology, vol. 17, p. e70035, 2024.
A. Mishra, A. Aggarwal, and F. Khan, "Medical Device-Associated Infections Caused by Biofilm-Forming Microbial Pathogens and Controlling Strategies," Antibiotics, vol. 13, p. 623, 2024.
X. Wang, C. Chen, J. Hu, C. Liu, Y. Ning, and F. Lu, "Current strategies for monitoring and controlling bacterial biofilm formation on medical surfaces," Ecotoxicology and Environmental Safety, vol. 282, p. 116709, 2024/09/01/ 2024.
V. P. P. Alonso, M. P. M. B. B. Gonçalves, F. A. E. de Brito, G. R. Barboza, L. d. O. Rocha, and N. C. C. Silva, "Dry surface biofilms in the food processing industry: An overview on surface characteristics, adhesion and biofilm formation, detection of biofilms, and dry sanitization methods," Comprehensive Reviews in Food Science and Food Safety, vol. 22, pp. 688-713, 2023.
F. A. Ansari, M. Jabeen, and I. Ahmad, "Pseudomonas azotoformans FAP5, a novel biofilm-forming PGPR strain, alleviates drought stress in wheat plant," International Journal of Environmental Science and Technology, vol. 18, pp. 3855-3870, 2021/12/01 2021.
N. Kalaria, A. Upadhyay, and A. Kumar, "Synthetic Compounds in Disruption of Salmonella Biofilm," in Salmonella Biofilms: Formation, Resistance, and Therapeutics, A. Kumar and A. Upadhyay, Eds., ed: Royal Society of Chemistry, 2025, p. 0.
I. Hashem and J. F. M. Van Impe, "The territorial nature of aggression in biofilms," Frontiers in Microbiology, vol. Volume 13 - 2022, 2022-August-23 2022.
Z. Lewandowsky and H. Beyenal, Foundamentals of Biofilm Research: CRC Press, 2007.
A. Kulshrestha and P. and Gupta, "Real-time biofilm detection techniques: advances and applications," Future Microbiology, vol. 19, pp. 1003-1016, 2024/07/23 2024.
D. Morgado, A. Fanesi, T. Martin, S. Tebbani, O. Bernard, and F. Lopes, "Non-destructive monitoring of microalgae biofilms," Bioresource Technology, vol. 398, p. 130520, 2024/04/01/ 2024.
S. Topçu, N. Tekçe, D. Kopuz, E. Y. Özcelik, F. Kolaylı, S. Tuncer, et al., "Effect of surface roughness and biofilm formation on the color properties of resin-infiltrated ceramic and lithium disilicate glass-ceramic CAD-CAM materials," The Journal of Prosthetic Dentistry, vol. 131, pp. 935.e1-935.e8, 2024/05/01/ 2024.
D. I. Avgoulas, M. Petala, R. Briandet, Y. Dergham, M.-F. Noirot-Gros, A. Konstantinidis, et al., "Effect of surface treatment and shear flow on biofilm formation over materials employed in space water storage and distribution systems," Frontiers in Materials, vol. Volume 11 - 2024, 2024-May-30 2024.
H.-T. Kim, G. Çakmak, Y.-H. Jo, E.-B. Jee, J.-H. Cho, H.-I. Yoon, et al., "Surface properties and biofilm formation on resins for subtractively and additively manufactured fixed dental prostheses aged in artificial saliva: Effect of material type and surface finishing," The Journal of Prosthetic Dentistry, vol. 133, pp. 594.e1-594.e9, 2025/02/01/ 2025.
F. Ebrahimi Tarki, M. Zarrabi, M. Sharbatdar, and A. A. Ali, "Physical parametric study of bacterial biofilm disruption and removal by jet impingement: A CFD investigation," Results in Engineering, vol. 23, p. 102490, 2024/09/01/ 2024.
X. Lyu, K. Liao, Z. Zou, G. He, and S. Liu, "Effects of Flow Velocity on Biofilm formation and corrosion behavior of L245 steel in the presence of sulfate reducing bacteria," in International Petroleum Technology Conference, 2024.
M. J. Romeu, J. M. Miranda, E. D. de Jong, J. Morais, V. Vasconcelos, J. Sjollema, et al., "Understanding the flow behavior around marine biofilms," Biofilm, vol. 7, p. 100204, 2024/06/01/ 2024.